Callus induction and somatic embryogenesis of Phalaenopsis

被引：104
|
作者：
Ishii, Y [1 ]
Takamura, T [1 ]
Goi, M [1 ]
Tanaka, M [1 ]
机构：
[1] Kagawa Univ, Fac Agr, Dept Hort, Miki, Kagawa 7610795, Japan
来源：
PLANT CELL REPORTS | 1998年 / 17卷 / 6-7期
关键词：
Phalaenopsis; protocorm-like bodies; callus induction; somatic embryogenesis;
D O I：
10.1007/s002990050423
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis Richard Shaffer 'Santa Cruz' were examined. Protocorm-like body (PLB) segments formed calli in Vacin and Went medium with sucrose. The optimal concentration of sucrose was 40 g.l(-1). Medium containing 200 ml.l(-1) coconut water together with 40 g.l(-1) sucrose was effective for callus induction. Gellan gum was suitable than agar as a gelling agent for callus induction. The calli easily formed PLBs after being transferred to a medium without sucrose. Histological observation suggested that the PLBs were somatic embryos. No variation was observed in the flowering plants regenerated through somatic embryogenesis.
引用
收藏
页码：446 / 450
页数：5
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