A highly multiplexed and sensitive RNA-seq protocol for simultaneous analysis of host and pathogen transcriptomes

被引：0

作者：

Roi Avraham

Nathan Haseley

Amy Fan

Zohar Bloom-Ackermann

Jonathan Livny

Deborah T Hung

机构：

[1] Broad Institute of MIT and Harvard,Department of Medicine

[2] Brigham and Women's Hospital,Department of Molecular Biology

[3] Harvard Medical School,Department of Microbiology and Immunobiology

[4] Massachusetts General Hospital,undefined

[5] Center for Computational and Integrative Biology,undefined

[6] Massachusetts General Hospital,undefined

[7] Harvard Medical School,undefined

[8] Present address: Department of Biological Regulation,undefined

[9] Weizmann Institute of Science,undefined

[10] Rehovot,undefined

[11] Israel.,undefined

来源：

Nature Protocols | 2016年 / 11卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

This protocol enables simultaneous analysis of host and bacterial transcripts by RNA-seq. It includes procedures for efficient host and bacterial cell lysis, barcoding of samples and analysis of both mammalian and microbial reads from mixed host–pathogen RNA-seq data.

引用

页码：1477 / 1491

页数：14

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