A highly multiplexed and sensitive RNA-seq protocol for simultaneous analysis of host and pathogen transcriptomes

被引：0
|
作者：
Roi Avraham
Nathan Haseley
Amy Fan
Zohar Bloom-Ackermann
Jonathan Livny
Deborah T Hung
机构：
[1] Broad Institute of MIT and Harvard,Department of Medicine
[2] Brigham and Women's Hospital,Department of Molecular Biology
[3] Harvard Medical School,Department of Microbiology and Immunobiology
[4] Massachusetts General Hospital,undefined
[5] Center for Computational and Integrative Biology,undefined
[6] Massachusetts General Hospital,undefined
[7] Harvard Medical School,undefined
[8] Present address: Department of Biological Regulation,undefined
[9] Weizmann Institute of Science,undefined
[10] Rehovot,undefined
[11] Israel.,undefined
来源：
Nature Protocols | 2016年 / 11卷
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摘要：
This protocol enables simultaneous analysis of host and bacterial transcripts by RNA-seq. It includes procedures for efficient host and bacterial cell lysis, barcoding of samples and analysis of both mammalian and microbial reads from mixed host–pathogen RNA-seq data.
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页码：1477 / 1491
页数：14
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