The LightCycler® 480 real-time PCR system: a versatile platform for genetic variation research

被引：0
|
作者：
Michael Hoffmann
Oliver Geulen
Christian Weilke
机构：
[1] Roche Applied Science,
来源：
Nature Methods | 2008年 / 5卷 / 3期
关键词：
D O I：
10.1038/nmeth.f.207
中图分类号：
学科分类号：
摘要：
Real-time PCR is a well established technique for studying genetic variation using various probe-based methods for genotyping as well as high-resolution analysis of whole amplicons melted in the presence of saturating DNA dyes. The latter, relatively new, method allows screening for unknown mutations or DNA modifications. The LightCycler® 480 real-time PCR system is a multiwell plate–based instrument that provides integrated applications for detecting and characterizing genetic variation using all these methodological approaches.
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