Fumaric Acid Production from Alkali-Pretreated Corncob by Fed-Batch Simultaneous Saccharification and Fermentation Combined with Separated Hydrolysis and Fermentation at High Solids Loading

被引:0
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作者
Xin Li
Jin Zhou
Shuiping Ouyang
Jia Ouyang
Qiang Yong
机构
[1] Nanjing Forestry University,Jiangsu Co
[2] Nanjing Forestry University,Innovation Center of Efficient Processing and Utilization of Forest Resources
[3] Nanjing Forestry University,Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education
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关键词
Fumaric acid; Alkali-pretreated corncob; High solids loading; Fed-batch simultaneous saccharification and fermentation;
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摘要
Production of fumaric acid from alkali-pretreated corncob (APC) at high solids loading was investigated using a combination of separated hydrolysis and fermentation (SHF) and fed-batch simultaneous saccharification and fermentation (SSF) by Rhizopus oryzae. Four different fermentation modes were tested to maximize fumaric acid concentration at high solids loading. The highest concentration of 41.32 g/L fumaric acid was obtained from 20 % (w/v) APC at 38 °C in the combined SHF and fed-batch SSF process, compared with 19.13 g/L fumaric acid in batch SSF alone. The results indicated that a combination of SHF and fed-batch SSF significantly improved production of fumaric acid from lignocellulose by R. oryzae than that achieved with batch SSF at high solids loading.
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页码:573 / 583
页数:10
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