Casitone-mediated expression of the prtP and prtM genes in Lactococcus lactis subsp. lactis BGIS29

被引：0

作者：

Natasa Miladinov

Oscar P. Kuipers

Ljubisa Topisirovic

机构：

[1] Institute of Molecular Genetics and Genetic Engineering,

[2] Vojvode Stepe 444a,undefined

[3] P.O.Box 446,undefined

[4] 11001 Belgrade,undefined

[5] Yugoslavia,undefined

[6] Microbial Ingredients Section,undefined

[7] NIZO Food Research,undefined

[8] P.O. Box 20,undefined

[9] 6710 BA Ede,undefined

[10] The Netherlands,undefined

[11] Present address: Molecular Genetics Group,undefined

[12] University of Groningen,undefined

[13] P.O.Box 14,undefined

[14] 9750 AA Haren,undefined

[15] The Netherlands,undefined

来源：

Archives of Microbiology | 2001年 / 177卷

关键词：

Lactococcus lactis Proteinase prtP gene prtM gene Regulation;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Casitone added to chemically defined medium (CDM) specifically influenced the regulation of the proteinase activity in the natural isolate Lactococcus lactis subsp. lactis BGIS29. Comparative analysis of the influence of casitone present in CDM on the proteolytic activity of strain BGIS29 and of L. lactis subsp. cremoris strains SK11 and Wg2 indicated that the proteolytic activity of strains BGIS29 and SK11 is casitone-dependent, whereas that of strain Wg2 is not. The regulatory region of the prt genes of strain BGIS29 was cloned and sequenced. The nucleotide sequence of the prt regulatory region of strain BGIS29 was distinctly different from that of L. lactis subsp. cremoris strains SK11 and Wg2. Transcriptional gene fusions with the Escherichia coli β-glucuronidase gene (gusA) were used to study medium-dependent expression of two divergent prtP and prtM promoters of strain BGIS29 (designated PprtP and PprtM, respectively). β-Glucuronidase assays and Northern blot analysis showed that the activities of PprtP and PprtM are controlled by casitone at the transcriptional level.

引用

页码：54 / 61

页数：7

共 26 条

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