Gene Cloning and Characterization of a Novel Highly Organic Solvent Tolerant Lipase from Proteus sp. SW1 and its Application for Biodiesel Production

被引：0
|
作者：
Wirongrong Whangsuk
Pareenart Sungkeeree
Sirinthra Thiengmag
Jarunee Kerdwong
Ratiboot Sallabhan
Skorn Mongkolsuk
Suvit Loprasert
机构：
[1] Chulabhorn Research Institute,Laboratory of Biotechnology
[2] Chulabhorn Graduate Institute,Applied Biological Sciences Program
[3] CHE,Center of Excellence on Environmental Health and Toxicology
[4] Ministry of Education,undefined
来源：
Molecular Biotechnology | 2013年 / 53卷
关键词：
Glycerol-ester hydrolase; Triacylglycerol acylhydrolase; Alkaline lipase; Biodiesel; Transesterification; Biofuel; Alcohol resistant;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Proteus sp. SW1 was found to produce an extracellular solvent tolerant lipase. The gene, lipA, encoding a bacterial lipase, was cloned from total Proteus sp. SW1 DNA. lipA was predicted to encode a 287 amino acid protein of 31.2 kDa belonging to the Group I proteobacterial lipases. Purified His-tagged LipA exhibited optimal activity at pH 10.0 and 55°C. It was highly stable in organic solvents retaining 112% of its activity in 100% isopropanol after 24 h, and exhibited more than 200% of its initial activity upon exposure to 60% acetone, ethanol, and hexane for 18 h. Biodiesel synthesis reactions, using a single step addition of 13% an acyl acceptor ethanol, showed that LipA was highly effective at converting palm oil into biodiesel.
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页码：55 / 62
页数：7
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