Investigating peste des petits ruminants (PPR) in naturally infected goats and sheep in Anseba Region, Eritrea, by reverse transcription polymerase chain reaction (RT-PCR)

被引：0

作者：

A. A. Oshiek

M. W. Abdelkadir

Bereket Mihreteab

S. B. Mengesha

G. H. Teklay

H. Yemane

H. G. Mesfun

G. H. Efrem

F. Njeumi

机构：

[1] Hamelmalo Agricultural College (HAC),

[2] National Animal and Plant Health Laboratory (NAPHL),undefined

[3] Food and Agriculture Organization of the United Nation,undefined

[4] FAO,undefined

来源：

Tropical Animal Health and Production | 2018年 / 50卷

关键词：

Peste des petits ruminants virus; Reverse transcriptase polymerase chain reaction; Anseba Region; Eritrea;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The impact of peste des petits ruminants (PPR) virus was investigated by reverse transcription polymerase chain reaction (RT-PCR) on different samples obtained from non-vaccinated diseased and necropsied sheep and goats showing PPR-like symptoms. The disease picture was typical and sheep were observed to be less susceptible. Nasal and rectal swabs, whole blood and pathological tissue samples from the lungs, intestine, and mesenteric lymph nodes were used for this study. The results of RT-PCR indicated that from a total of 32 samples collected, 12 (41%) were positive by this method. Out of those collected samples, 29 were from goats and 3 were from sheep. Nasal and rectal swabs and blood samples were superior in detection of the PPR virus compared to other tissue samples.

引用

页码：915 / 920

页数：5

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