A novel membrane based process to isolate photosystem-I membrane complex from spinach

被引：0
|
作者：
Jianguo Liu
Mengmeng Yin
Meng Wang
Xuefang Zhang
Baosheng Ge
Shuang Liu
Jianren Lu
Zhanfeng Cui
机构：
[1] China University of Petroleum (East China),Center for Bioengineering and Biotechnology
[2] Manchester University,School of Physics
[3] University of Oxford,Department of Engineering Science
来源：
Photosynthesis Research | 2011年 / 107卷
关键词：
Ultrafiltration; Photosystem-I; Membrane protein; Spinach; Activity;
D O I：
暂无
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学科分类号：
摘要：
The isolation of photosystem-I (PS-I) from spinach has been conducted using ultrafiltration with 300 kDa molecular weight cut-off polyethersulfone membranes. The effects of ultrafiltration operating conditions on PS-I activity were optimized using parameter scanning ultrafiltration. These conditions included solution pH, ionic strength, stirring speed, and permeate flux. The effects of detergent (Triton X-100 and n-dodecyl-beta-D-maltoside) concentration on time dependent activity of PS-I were also studied using an O2 electrode. Under optimized conditions, the PS-I purity obtained in the retentate was about 84% and the activity recovery was greater than 94% after ultrafiltration. To our knowledge, this is the first report of the isolation of a membrane protein using ultrafiltration alone.
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页码：187 / 193
页数：6
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