Genome editing in plants by engineered CRISPR–Cas9 recognizing NG PAM

被引：0

作者：

Masaki Endo

Masafumi Mikami

Akira Endo

Hidetaka Kaya

Takeshi Itoh

Hiroshi Nishimasu

Osamu Nureki

Seiichi Toki

机构：

[1] National Agriculture and Food Research Organization,Plant Genome Engineering Research Unit, Institute of Agrobiological Sciences

[2] Yokohama City University,Graduate School of Nanobioscience

[3] National Agriculture and Food Research Organization,Bioinformatics Team, Advanced Analysis Center

[4] The University of Tokyo,Graduate School of Science, Department of Biological Sciences

[5] Yokohama City University,Kihara Institute for Biological Research

[6] Kaneka Corporation,Biotechnology Development Laboratories

[7] Ehime University,Graduate School of Agriculture

来源：

Nature Plants | 2019年 / 5卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Streptococcus pyogenes Cas9 (SpCas9) is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1) can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5′ end of the target sequence.

引用

页码：14 / 17

页数：3

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