Genome-wide base-resolution mapping of DNA methylation in single cells using single-cell bisulfite sequencing (scBS-seq)

被引：0

作者：

Stephen J Clark

Sébastien A Smallwood

Heather J Lee

Felix Krueger

Wolf Reik

Gavin Kelsey

机构：

[1] Epigenetics Programme,Department of Physiology

[2] Babraham Institute,undefined

[3] Wellcome Trust Sanger Institute,undefined

[4] Hinxton,undefined

[5] Bioinformatics Group,undefined

[6] Babraham Institute,undefined

[7] Centre for Trophoblast Research,undefined

[8] University of Cambridge,undefined

[9] Development and Neuroscience,undefined

[10] University of Cambridge,undefined

[11] Present address: Friedrich Miescher Institute for Biomedical Research,undefined

[12] Basel,undefined

[13] Switzerland (S.A.S.); School of Biomedical Sciences and Pharmacy,undefined

[14] University of Newcastle,undefined

[15] Newcastle,undefined

[16] Australia (H.J.L.).,undefined

来源：

Nature Protocols | 2017年 / 12卷

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摘要：

Single-cell bisulfite sequencing (scBS-seq) is a cost-effective method for surveying CpG methylation across genomes of single cells. It gives higher coverage than other methods, is automatable and is compatible with parallel RNA-seq from the same cell.

引用

页码：534 / 547

页数：13

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