Genome-wide base-resolution mapping of DNA methylation in single cells using single-cell bisulfite sequencing (scBS-seq)

被引：0

作者：

Stephen J Clark

Sébastien A Smallwood

Heather J Lee

Felix Krueger

Wolf Reik

Gavin Kelsey

机构：

[1] Epigenetics Programme,Department of Physiology

[2] Babraham Institute,undefined

[3] Wellcome Trust Sanger Institute,undefined

[4] Hinxton,undefined

[5] Bioinformatics Group,undefined

[6] Babraham Institute,undefined

[7] Centre for Trophoblast Research,undefined

[8] University of Cambridge,undefined

[9] Development and Neuroscience,undefined

[10] University of Cambridge,undefined

[11] Present address: Friedrich Miescher Institute for Biomedical Research,undefined

[12] Basel,undefined

[13] Switzerland (S.A.S.); School of Biomedical Sciences and Pharmacy,undefined

[14] University of Newcastle,undefined

[15] Newcastle,undefined

[16] Australia (H.J.L.).,undefined

来源：

Nature Protocols | 2017年 / 12卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Single-cell bisulfite sequencing (scBS-seq) is a cost-effective method for surveying CpG methylation across genomes of single cells. It gives higher coverage than other methods, is automatable and is compatible with parallel RNA-seq from the same cell.

引用

页码：534 / 547

页数：13

共 50 条

[1] Genome-wide base-resolution mapping of DNA methylation in single cells using single-cell bisulfite sequencing( scBS-seq)
Clark, Stephen J.
Smallwood, Sebastien A.
Lee, Heather J.
Krueger, Felix
Reik, Wolf
Kelsey, Gavin
NATURE PROTOCOLS, 2017, 12 (03) : 534 - U159
[2] Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity
Smallwood, Sebastien A.
Lee, Heather J.
Angermueller, Christof
Krueger, Felix
Saadeh, Heba
Peet, Julian
Andrews, Simon R.
Stegle, Oliver
Reik, Wolf
Kelsey, Gavin
NATURE METHODS, 2014, 11 (08) : 817 - 820
[3] Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity
Smallwood S.A.
Lee H.J.
Angermueller C.
Krueger F.
Saadeh H.
Peat J.
Andrews S.R.
Stegle O.
Reik W.
Kelsey G.
Nature Methods, 2014, 11 (8) : 817 - 820
[4] Mapping replication timing domains genome wide in single mammalian cells with single-cell DNA replication sequencing
Hisashi Miura
Saori Takahashi
Takahiro Shibata
Koji Nagao
Chikashi Obuse
Katsuzumi Okumura
Masato Ogata
Ichiro Hiratani
Shin-ichiro Takebayashi
Nature Protocols, 2020, 15 : 4058 - 4100
[5] Single-cell genome-wide bisulfite sequencing uncovers extensive heterogeneity in the mouse liver methylome
Gravina, Silvia
Dong, Xiao
Yu, Bo
Vijg, Jan
GENOME BIOLOGY, 2016, 17
[6] Single-cell genome-wide bisulfite sequencing uncovers extensive heterogeneity in the mouse liver methylome
Silvia Gravina
Xiao Dong
Bo Yu
Jan Vijg
Genome Biology, 17
[7] Mapping replication timing domains genome wide in single mammalian cells with single-cell DNA replication sequencing
Miura, Hisashi
Takahashi, Saori
Shibata, Takahiro
Nagao, Koji
Obuse, Chikashi
Okumura, Katsuzumi
Ogata, Masato
Hiratani, Ichiro
Takebayashi, Shin-ichiro
NATURE PROTOCOLS, 2020, 15 (12) : 4058 - 4100
[8] ZIP-seq: genome-wide mapping of trinucleotide repeats at single-base resolution
Xu, Xingxing
Tao, Yonghui
Fu, Xing
Yu, Tao
Li, Yuanyuan
Chen, Kan
Ding, Xiaoyan
Ruan, Kangcheng
Jing, Naihe
Hu, Ronggui
JOURNAL OF MOLECULAR CELL BIOLOGY, 2014, 6 (01) : 93 - 96
[9] Rat genome-wide capture of gene regulatory regions and base-resolution quantitation of DNA methylation
Freeman, Willard
Stanford, David
Masser, Dustin
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2015, 56 (07)
[10] Analysis of population heterogeneity in CHO cells by genome-wide DNA methylation analysis and by multi-modal single-cell sequencing
Boehl, Elias
Raddatz, Guenter
Roy, Suki
Huang, Lingzhi
Sandhu, Jasrene Kaur
Igwe, Emeka Ignatius
Rodriguez-Paredes, Manuel
Boehl, Florian
Lyko, Frank
JOURNAL OF BIOTECHNOLOGY, 2024, 396 : 72 - 79

← 1 2 3 4 5 →