Characteristics of pullulanases from extremely thermophilic archaea isolated from deep-sea hydrothermal vents

被引：0
|
作者：
Hubert Gantelet
Christine Ladrat
Anne Godfroy
Georges Barbier
Francis Duchiron
机构：
[1] Université de Reims-Champagne-Ardenne,Laboratoire de Microbiologie Industrielle
[2] UFR Sciences Exactes et Naturelles,IFREMER, Centre de Brest
[3] Laboratoire de Biotechnologie des Micro-organismes Hydrothermaux,undefined
来源：
Biotechnology Letters | 1998年 / 20卷
关键词：
pullulanase; deep-sea hydrothermal vents; Thermococcus; thermostability; archaea.;
D O I：
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中图分类号：
学科分类号：
摘要：
Two out of three extremely thermophilic anaerobic archaea, isolated from deep-sea hydrothermal vents, produced pullulanase activity in the presence of maltose in the growth medium. Enzyme activities were mainly extracellular and characterized by optimum temperatures of 95°C and 80–95°C, optimum pH of 5.0–7.0 and a high degree of thermostability. One strain when grown in a fermenter with maltose as inducer produced pullulanase at 35 U/l. © Rapid Science Ltd. 1998
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页码：819 / 823
页数：4
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