Identification of AFLP markers closely linked to the rhm gene for resistance to Southern Corn Leaf Blight in maize by using bulked segregant analysis

被引：0
|
作者：
H.-w. Cai
Z.-s. Gao
N. Yuyama
N. Ogawa
机构：
[1] Japan Grassland Farming and Forage Seed Association,Forage Crop Research Institute
[2] Kansas State University,Department of Agronomy
来源：
Molecular Genetics and Genomics | 2003年 / 269卷
关键词：
Southern Corn Leaf Blight (SCLB); Resistance gene; Amplified fragment length polymorphism (AFLP); Bulked segregant analysis (BSA);
D O I：
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中图分类号：
学科分类号：
摘要：
Southern Corn Leaf Blight (SCLB) is an important disease in warm-temperate and tropical corn-producing areas throughout the world. We applied a combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) to a large F2 population in order to identify molecular markers linked to the rhm gene for resistance to SCLB. One co-dominant AFLP marker, p7m36, was mapped to a position 1.0 cM from rhm, and we converted this marker to an STS (sequence-tagged site) marker. Combined with the previously identified agrP144, this new marker may be useful for map-based cloning of the rhm gene and marker-assisted selection for rhm.
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页码：299 / 303
页数：4
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