The Czech Republic has a northern hemisphere Atlantic-continental type of moderate climate. Mean annual temperature ranges between 1.0 and 9.4 °C (between 8.8 and 18.5 °C in summer and between –6.8 and 0.2 °C in winter). Annual precipitation ranges between 450 mm in dry regions and 1300 mm in mountainous regions of the country. With its 2000 m3 per capita fresh water availability, the Czech Republic is slightly below average. Occasional water shortages do not usually result from general unavailability of water resources but rather from time or space variability of water supply/demand and high degree of water resources exploitation. To study potential impacts of climate change on hydrological system and water resources, four river basins have been selected in the territory of the Czech Republic: the Elbe River at Decin (50761.7 km2), the Zelivka River at Soutice (1188.6 km2), the Upa River at Ceska Skalice (460.7 km2) and the Metuje River at Marsov n. M. (93.9 km2). To simulate potential changes in runoff, three hydrological models have been applied using incremental and GCM (GISS, GFDL and CCCM) scenarios: the BILAN water balance model, the SACRAMENTO (SAC-SMA) conceptual model and the CLIRUN water balance model. The paper reviews methods applied in the study, results of the assessments and concludes with suggestions for possible general adaptation policy options where the preference is for nonstructural measures such as water conservation, efficient water demand management and protection of water resources.