Optimized labeling of membrane proteins for applications to super-resolution imaging in confined cellular environments using monomeric streptavidin

被引：0

作者：

Ingrid Chamma

Olivier Rossier

Grégory Giannone

Olivier Thoumine

Matthieu Sainlos

机构：

[1] Interdisciplinary Institute for Neuroscience,

[2] UMR 5297,undefined

[3] Centre National de la Recherche Scientifique,undefined

[4] Interdisciplinary Institute for Neuroscience,undefined

[5] University of Bordeaux,undefined

来源：

Nature Protocols | 2017年 / 12卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

This protocol describes how to express and purify a protein probe and subsequently conjugate the probe to dyes with different fluorescence properties. The probes have applications in super-resolution imaging.

引用

页码：748 / 763

页数：15

共 30 条

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