Optimized labeling of membrane proteins for applications to super-resolution imaging in confined cellular environments using monomeric streptavidin

被引：0
|
作者：
Ingrid Chamma
Olivier Rossier
Grégory Giannone
Olivier Thoumine
Matthieu Sainlos
机构：
[1] Interdisciplinary Institute for Neuroscience,
[2] UMR 5297,undefined
[3] Centre National de la Recherche Scientifique,undefined
[4] Interdisciplinary Institute for Neuroscience,undefined
[5] University of Bordeaux,undefined
来源：
Nature Protocols | 2017年 / 12卷
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学科分类号：
摘要：
This protocol describes how to express and purify a protein probe and subsequently conjugate the probe to dyes with different fluorescence properties. The probes have applications in super-resolution imaging.
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页码：748 / 763
页数：15
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