Effects of 17β-estradiol on the expression of IL-6, IL-11 and NF-κB in human MG-63 osteoblast-like cell line

被引：1
|
作者：
Wang Yunlin
Liao Eyuan
Xiang Guangda
Dai Ruchun
Xiao Xinhua
Luo Xianghang
机构：
[1] Huazhong university of Science and Technology,Department of Gerontism, Tongji Hospital, Tongji Medical College
[2] Central South University,Institute of Endocrinology and Metabolism, Second Xiangya Hospital
[3] Wuhan General Hospital of PLA,Department of Endocrinology
来源：
Journal of Huazhong University of Science and Technology [Medical Sciences] | 2006年 / 26卷 / 1期
关键词：
IL-6; IL-11; NF κB; MG-63;
D O I：
10.1007/BF02828038
中图分类号：
学科分类号：
摘要：
In order to characterize the effects of 17β estradiol (17β-E2) on the expression of IL-6, IL 11 and NF-κB in the human MG 63 osteoblast like cell line, the expression of IL-6 was detected by RT-PCR. Northern blot and Western blot. The expression of IL-11 was determined by RT-PCR, and NF-κB by Western blot. The results showed that 17β-E2 down-regulated the expression of IL 6 mRNA and protein. IL 11 mRNA and NF κB protein in MG-63 cells. It was suggested that the expression of NF-κB, IL-6 and IL-11 in MG 63 cells could be suppressed by 17β-E2, and this might lend support to estrogen replacement therapy in postmenopausal women.
引用
收藏
页码：53 / 58
页数：5
相关论文
共 50 条
  • [1] Effects of 17β-Estradiol on the Expression of IL-6, IL-11 and NF-κB in Human MG-63 Osteoblast-like Cell Line
    王运林
    廖二元
    向光大
    戴如春
    肖新华
    罗湘杭
    华中科技大学学报(医学英德文版), 2006, (01) : 53 - 58
  • [2] Regulation of TNF-α-induced IL-6 production in MG-63 human osteoblast-like cells
    Webb, SJ
    McPherson, JR
    Pahan, K
    Koka, S
    JOURNAL OF DENTAL RESEARCH, 2002, 81 (01) : 17 - 22
  • [3] Selective effect of thiazides on the human osteoblast-like cell line MG-63
    Aubin, R
    Menard, P
    Lajeunesse, D
    KIDNEY INTERNATIONAL, 1996, 50 (05) : 1476 - 1482
  • [4] Divergent effects of interleukin (IL)-6 and IL-11 on the concentrations of glucocorticoid receptor in human osteoblast-like cells
    Dovio, A
    Sartori, ML
    Masera, RG
    Ceoloni, B
    Prolo, P
    Racca, S
    Chiappelli, F
    Angeli, A
    OSTEOPOROSIS INTERNATIONAL, 2002, 13 : S99 - S99
  • [5] 17β-雌二醇对人骨肉瘤MG-63细胞株IL-6、IL-11和NF-κB表达的作用
    王运林
    廖二元
    戴如春
    向光大
    肖新华
    罗湘杭
    中国现代医学杂志, 2005, (24) : 3710 - 3714
  • [6] Divergent effects of interleukin (IL)-6 and IL-11 on glucocorticoid receptor number in human osteoblast-like cells.
    Dovio, A
    Sartori, ML
    Ceoloni, B
    Saba, L
    Prolo, P
    Chiappelli, F
    Angeli, A
    JOURNAL OF BONE AND MINERAL RESEARCH, 2002, 17 : S449 - S449
  • [7] Action potentials in primary osteoblasts and in the MG-63 osteoblast-like cell line
    Pangalos, Maria
    Bintig, Willem
    Schlingmann, Barbara
    Feyerabend, Frank
    Witte, Frank
    Begandt, Daniela
    Heisterkamp, Alexander
    Ngezahayo, Anaclet
    JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 2011, 43 (03) : 311 - 322
  • [8] Action potentials in primary osteoblasts and in the MG-63 osteoblast-like cell line
    Maria Pangalos
    Willem Bintig
    Barbara Schlingmann
    Frank Feyerabend
    Frank Witte
    Daniela Begandt
    Alexander Heisterkamp
    Anaclet Ngezahayo
    Journal of Bioenergetics and Biomembranes, 2011, 43 : 311 - 322
  • [9] Inhibitory effect of physiological concentrations of cortisol but not estradiol on interleukin (IL)-6 production by human osteoblast-like cell lines with different constitutive IL-6 expression
    Dovio, A
    Sartori, ML
    Masera, RG
    Racca, S
    Angeli, A
    CYTOKINE, 2001, 15 (01) : 47 - 52
  • [10] Effect of Steroids and Sulfated Steroids on Growth of the Human MG-63 Osteoblast-like Cell Line.
    Selcer, Kyle W.
    Vollmer, Laura L.
    BIOLOGY OF REPRODUCTION, 2010, : 143 - 143
12345