Rapid Method for Measurement of Acetylcholinesterase Activity

被引：0
|
作者：
A. M. Antokhin
E. T. Gainullina
S. B. Ryzhikov
V. F. Taranchenko
D. K. Yavaeva
机构：
[1] Ministry of Defense of the Russian Federation,Research Center
来源：
Bulletin of Experimental Biology and Medicine | 2009年 / 147卷
关键词：
acetylcholinesterase; fluorogenic label; fluorescence;
D O I：
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学科分类号：
摘要：
Fluorescence intensity of thioflavin T fluorogenic label increased significantly as a result of formation of enzyme-inhibitory complex with acetylcholinesterase of human blood erythrocytes. Thioflavin T is a reversible inhibitor, selectively reacting with acetylcholinesterase. Thioflavin T fluorescence intensity is proportional to acetylcholinesterase activity for the studied interval of enzyme activities. A rapid fluorescent method for measuring acetylcholinesterase activity is proposed.
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页码：109 / 110
页数：1
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