Skin whitening activity of supercritical fluid extract from spent coffee in B16F10 melanoma cell

In the present study, we evaluated the skin whitening effects of supercritical fluid extract (SFE) of the spent coffee (SC) on tyrosinase inhibition activity, melanin synthesis inhibition activity and DOPA staining. Hot water extracts from coffee bean (CB) and SC and drip extracts from CB were employed for control. Inhibitions of melanin activities of SFE (SC) at short processing time were significantly higher than other values under the same extract processing. In comparison, inhibition of melanin activities appeared at high pressure in CB. Tyrosinase inhibition activity of SFE (SC) extracted for 1 h at 150 bar was significantly higher than those of SFE (CB and SC) even of controls. According to the above results, SFE (SC) extracted for 2 h or less at 450 bar showed significantly high activities in the melanin synthesis inhibition and tyrosinase inhibition.