High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells

被引：0
|
作者：
Volker Jäger
Konrad Büssow
Andreas Wagner
Susanne Weber
Michael Hust
André Frenzel
Thomas Schirrmann
机构：
[1] Helmholtz-Zentrum für Infektionsforschung GmbH,Department of Molecular Structural Biology
[2] Institute of Biochemistry,Department of Biotechnology, Technische Universität Braunschweig
[3] Biotechnology and Bioinformatics,undefined
来源：
BMC Biotechnology | / 13卷
关键词：
Recombinant Antibodies; Single Chain Fv; scFv-Fc; ImmunoRNase; Transient Mammalian Protein Production; Serum-free medium;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
引用
收藏
相关论文
共 50 条
  • [21] Inhibitory effect of biochanin a on cAMP level in HEK293 cells
    Yoon, Y. C.
    Hwang, J. T.
    Sung, M. J.
    Wang, S.
    Park, J. H.
    PLANTA MEDICA, 2012, 78 (11) : 1245 - 1245
  • [22] Transient gene expression in HEK293 cells: Peptone addition posttransfection improves recombinant protein synthesis
    Pham, PL
    Perret, S
    Cass, B
    Carpentier, E
    St-Laurent, G
    Bisson, L
    Kamen, A
    Durocher, Y
    BIOTECHNOLOGY AND BIOENGINEERING, 2005, 90 (03) : 332 - 344
  • [23] Unraveling Cytotoxicity in HEK293 Cells During Recombinant AAV Production for Gene Therapy Applications
    Ladiwala, Pranay
    Ndahiro, Nelson
    Hauk, Pricila
    Wen, Junneng
    Sargunas, Justin
    Chen, Yu-Ju
    Barton, Erik
    Betenbaugh, Michael J.
    BIOTECHNOLOGY JOURNAL, 2025, 20 (03)
  • [24] HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors
    Tan, Evan
    Chin, Cara Sze Hui
    Lim, Zhi Feng Sherman
    Ng, Say Kong
    FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2021, 9
  • [25] Interferon inhibitors increase rAAV production in HEK293 cells
    Wang, Yongdan
    Fu, Qiang
    Sha, Sha
    Yoon, Seongkyu
    JOURNAL OF BIOTECHNOLOGY, 2025, 399 : 9 - 18
  • [26] Transient gene expression in HEK293 and vero cells immobilised on microcarriers
    Fliedl, Lukas
    Kaisermayer, Christian
    JOURNAL OF BIOTECHNOLOGY, 2011, 153 (1-2) : 15 - 21
  • [27] A robust transfection reagent for the transfection of CHO and HEK293 cells and production of recombinant proteins and lentiviral particles-PTG1
    Goncalves, Cristine
    Gross, Fabian
    Guegan, Philippe
    Cheradame, Herve
    Midoux, Patrick
    BIOTECHNOLOGY JOURNAL, 2014, 9 (11) : 1380 - 1388
  • [28] The CAG promoter maintains high-level transgene expression in HEK293 cells
    Dou, Yuanyuan
    Lin, Yan
    Wang, Tian-yun
    Wang, Xiao-Yin
    Jia, Yan-long
    Zhao, Chun-peng
    FEBS OPEN BIO, 2021, 11 (01): : 95 - 104
  • [29] Optimising the transient expression of GABA(A) receptors in adherent HEK293 cells
    Devenish, Steven O.
    Johnston, Graham A. R.
    Chebib, Mary
    Hanrahan, Jane R.
    PROTEIN EXPRESSION AND PURIFICATION, 2019, 154 : 7 - 15
  • [30] Hek293 as a recombinant protein factory: three different approaches for protein production
    Leticia Liste-Calleja
    Martí Lecina
    Roland Schucht
    Dagmar Wirth
    Hansjörg Hauser
    Jordi J Cairó
    BMC Proceedings, 9 (Suppl 9)
12345