Crown ether activates cholesterol oxidase in low water media

被引：0
|
作者：
Inna K. Sakodinskaya
Alexander D. Ryabov
机构：
[1] Moscow State University,Chemistry Department
来源：
Biotechnology Letters | 2000年 / 22卷
关键词：
cholesterol oxidase; crown ether; enzyme reactivity; propan-2-ol;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Kinetic studies of cholesterol oxidase-catalysed oxidation of cholesterol in water/2-propanol mixtures showed a decrease of Vmax/Km values on the increase of concentration of the organic co-solvent. Addition of 18-crown-6 to the reaction medium results in an increase of Vmax up to 16 times, and Vmax/Km up to 8.4 times, enhancing the activity of cholesterol oxidase in 2-propanol/water (88:12 v/v) to 3.5 times compared to the level observed in 46% 2-propanol.
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页码：173 / 176
页数：3
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