Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain

被引:0
|
作者
Victor H Obungu
A Lee Burns
Sunita K Agarwal
Settara C Chandrasekharapa
Robert S Adelstein
Stephen J Marx
机构
[1] Metabolic Diseases Branch,
[2] National Institute of Diabetes and Digestive and Kidney Diseases,undefined
[3] National Institutes of Health,undefined
[4] Genome Technology Branch,undefined
[5] National Human Genome Research Institute,undefined
[6] National Institutes of Health,undefined
[7] Laboratory of Molecular Cardiology,undefined
[8] National Heart,undefined
[9] Lung,undefined
[10] and Blood Institute,undefined
[11] National Institutes of Health,undefined
来源
Oncogene | 2003年 / 22卷
关键词
MEN1; menin; TAP; nonmuscle myosin; cytokinesis;
D O I
暂无
中图分类号
学科分类号
摘要
MEN1 is a likely tumor suppressor gene that encodes a novel protein, menin. Menin is a 610 amino-acid residue protein with as yet unknown function(s). We have used tandem affinity purification and mass spectroscopy to isolate and identify proteins associating with menin from cultured HeLa cell extracts. This strategy has resulted in the isolation and identification of nonmuscle myosin type II-A heavy chain (NMHC II-A) as a menin interacting protein. This interaction was confirmed by glutathione-S-transferase pulldown assays, by coimmunoprecipitation, and by actin selection of myosin. We have further identified the amino-terminal region of menin and the head domain of NMHC II-A to be regions required for this interaction. Moreover menin was seen to colocalize with this myosin isoform in the cleavage furrow of dividing cells by indirect immunofluoresence. These data indicate that menin through binding to NMHC II-A could participate in cell division and in other processes that involve NMHC II-A.
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页码:6347 / 6358
页数:11
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