Global site-specific analysis of glycoprotein N-glycan processing

被引：0
|
作者：
Liwei Cao
Jolene K Diedrich
Yuanhui Ma
Nianshuang Wang
Matthias Pauthner
Sung-Kyu Robin Park
Claire M Delahunty
Jason S McLellan
Dennis R Burton
John R Yates
James C Paulson
机构：
[1] The Scripps Research Institute,Department of Molecular Medicine
[2] Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery and IAVI Neutralizing Antibody Center,Department of Immunology and Microbiology
[3] The Scripps Research Institute,Department of Biochemistry and Cell Biology
[4] Geisel School of Medicine at Dartmouth,undefined
[5] Ragon Institute of MGH,undefined
[6] MIT and Harvard,undefined
来源：
Nature Protocols | 2018年 / 13卷
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摘要：
This protocol describes a semiquantitative glycoproteomics method using sequential treatment with endoglycosidases to create unique mass signatures to determine glycan occupancy and proportion of high-mannose and complex glycans at each glycosite.
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页码：1196 / 1212
页数：16
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