Different feeding strategy for the production of biosurfactant from Pseudomonas aeruginosa USM AR2 in modified bioreactor

被引:1
|
作者
M. S. Salwa
M. N. Nur Asshifa
A. A. Amirul
Ahmad R. M. Yahya
机构
[1] Universiti Sains Malaysia,School of Biological Sciences
来源
Biotechnology and Bioprocess Engineering | 2009年 / 14卷
关键词
rhamnolipid; biosurfactant; feeding strategy; foam collector; bioreactor;
D O I
暂无
中图分类号
学科分类号
摘要
A locally-isolated Pseudomonas aeruginosa USM AR2 possessing the ability to produce glycolipid-type biosurfactant (rhamnolipid) was used in this research to explore fermentation technology for rhamnolipid production. Rhamnolipid concentration in 2.5 L fed-batch fermentation was improved from 0.173 to 8.06 g/L by manipulating the feeding strategy and cultivation protocol. The culture was fed with petroleum diesel and complex medium. The highest rhamnolipid concentration was achieved when the culture was initially fed with both petroleum diesel and complex medium, followed by feeding of petroleum diesel only at the end of the stationary phase. Severe foaming problem was resolved by modifying and integrating a foam recycler to the bioreactor. This successfully extended the cultivation period and increased the yield of final rhamnolipid. No antifoam agent was added as this modified bioreactor allowed cultivation to proceed even under foam generation. The viscosity measurement, surface tension activity test, and drop-collapse test were performed as an indirect measure of rhamnolipid presence.
引用
收藏
页码:763 / 768
页数:5
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