Modulation of glutathione level during butyrate-induced differentiation in human colon derived HT-29 cells

被引：0
|
作者：
Outhiriaradjou Benard
K.A. Balasubramanian
机构：
[1] Christian Medical College and Hospital,The Wellcome Trust Research Laboratory, Department of Gastrointestinal Sciences
来源：
Molecular and Cellular Biochemistry | 1997年 / 170卷
关键词：
GSH; butyrate; BSO; HT-29 cells; proliferation; differentiation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Glutathione plays an important role in various cellular functions including cell growth and differentiation. In the present study, cell differentiation was induced by butyrate in human colon cell line HT-29 and cellular thiol status was assessed. It was observed that butyrate-induced differentiation was associated with decrease in cellular GSH level and this was prominent at early stages of differentiation. Buthionine sulfoximine (BSO), a specific cellular GSH depleting agent, did not induce differentiation in cells but potentiated the differentiation induced by butyrate. Both BSO and butyrate individually and together inhibited cell growth. These studies suggest that cellular GSH level is modulated in butyrate-induced differentiation and decrease of GSH at the initial stage might facilitate cellular differentiation.
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页码：109 / 114
页数：5
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