Engineering and expression of a recombinant fusion protein possessing fibroblast growth factor-2 and fibronectin fragment

被引：0
|
作者：
Jun-Hyeog Jang
Chong-Pyoung Chung
机构：
[1] Inha University College of Medicine,Department of Biochemistry
[2] Seoul National University College of Dentistry,Intellectual Biointerface Engineering Center and Department of Periodontology
来源：
Biotechnology Letters | 2004年 / 26卷
关键词：
cell adhesion; extracellular matrix; fibroblast growth factor; fibronectin; fusion;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
There is a synergistic effect between fibronectin and fibroblast growth factor 2 (FGF-2) on osteoblast cell adhesion through an extracellular, signal-regulated kinase pathway. Here we describe the engineering of a fusion protein containing fibronectin fragment (FNIII9-10) connected to the COOH-terminus of FGF-2. Purified FGF2-FNIII9-10 fusion protein exhibited a significant increase of cell adhesion and proliferation of MG63 cells compared with FNIII9-10 alone (p < 0.05).
引用
收藏
页码：1837 / 1840
页数：3
相关论文
共 50 条
  • [41] Fibroblast Growth Factor-2 Signaling in Neurogenesis and Neurodegeneration
    Woodbury, Maya E.
    Ikezu, Tsuneya
    JOURNAL OF NEUROIMMUNE PHARMACOLOGY, 2014, 9 (02) : 92 - 101
  • [42] Differential expression of fibroblast growth factor-2 and fibroblast growth factor receptor 1 in a scarring and nonscarring model of CNS injury in the rat
    Smith, C
    Berry, M
    Clarke, WE
    Logan, A
    EUROPEAN JOURNAL OF NEUROSCIENCE, 2001, 13 (03) : 443 - 456
  • [43] Action of fibroblast growth factor-2 on the intervertebral disc
    Li, Xin
    An, Howard S.
    Ellman, Michael
    Phillips, Frank
    Thonar, Eugene J.
    Park, Daniel K.
    Udayakumar, Ranjith K.
    Im, Hee-Jeong
    ARTHRITIS RESEARCH & THERAPY, 2008, 10 (02)
  • [44] Action of fibroblast growth factor-2 on the intervertebral disc
    Xin Li
    Howard S An
    Michael Ellman
    Frank Phillips
    Eugene J Thonar
    Daniel K Park
    Ranjith K Udayakumar
    Hee-Jeong Im
    Arthritis Research & Therapy, 10
  • [45] New insights in the biology of fibroblast growth factor-2
    Andreas Bikfalvi
    Catherine Savona
    Catherine Perollet
    Sophie Javerzat
    Angiogenesis, 1997, 1 (2) : 155 - 173
  • [46] Characterization of fibroblast growth factor-2 binding to ribosomes
    Klein, S
    Morimoto, T
    Rifkin, DB
    GROWTH FACTORS, 1996, 13 (3-4) : 219 - 228
  • [47] Fibroblast growth factor-2 alters the nature of extinction
    Graham, Bronwyn M.
    Richardson, Rick
    LEARNING & MEMORY, 2011, 18 (02) : 80 - 84
  • [48] Altered expression of vascular endothelial growth factor, fibroblast growth factor-2 and endostatin in patients with hepatocellular carcinoma
    Uematsu, S
    Higashi, T
    Nouso, K
    Kariyama, K
    Nakamura, SI
    Suzuki, M
    Nakatsukasa, H
    Kobayashi, Y
    Hanafusa, T
    Tsuji, T
    Shiratori, Y
    JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2005, 20 (04) : 583 - 588
  • [49] Expression of human fibroblast growth factor-2 and cell transformation: Some paradoxical aspects
    Amalric, F
    Galy, B
    Couderc, B
    Maret, A
    Prats, AC
    Prats, H
    ANTIBODY IMMUNOCONJUGATES AND RADIOPHARMACEUTICALS, 1995, 8 (04): : 243 - 250
  • [50] Influence of maternal diabetes on placental fibroblast growth factor-2 expression, proliferation, and apoptosis
    Burleigh, DW
    Stewart, K
    Grindle, KM
    Kay, HH
    Golos, TG
    JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION, 2004, 11 (01) : 36 - 41
12345