Mili and Miwi target RNA repertoire reveals piRNA biogenesis and function of Miwi in spermiogenesis

被引：0

作者：

Anastassios Vourekas

Qi Zheng

Panagiotis Alexiou

Manolis Maragkakis

Yohei Kirino

Brian D Gregory

Zissimos Mourelatos

机构：

[1] Perelman School of Medicine,Department of Pathology and Laboratory Medicine, Division of Neuropathology

[2] University of Pennsylvania,Department of Biology

[3] University of Pennsylvania,Department of Biomedical Sciences

[4] PENN Genome Frontiers Institute,undefined

[5] University of Pennsylvania,undefined

[6] Cedars-Sinai Medical Center,undefined

[7] Genomics and Computational Biology Graduate Program,undefined

[8] University of Pennsylvania,undefined

来源：

Nature Structural & Molecular Biology | 2012年 / 19卷

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摘要：

Piwi proteins bind a class of small germline RNAs, called piRNAs, whose biogenesis and functions are largely unknown. High-throughput sequencing after cross-linking and immunoprecipitation (HITS-CLIP) analysis in combination with RNA sequencing to analyze the genome-wide RNA target repertoire of mouse Piwi (Miwi) proteins in testis uncovers the in vivo piRNA biogenesis pathway and demonstrates that Miwi binds spermiogenic RNAs directly without using piRNAs as guides.

引用

页码：773 / 781

页数：8