Transfection of mammalian cells by electroporation

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10.1038/nmeth0106-67
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Pulsed electrical fields can be used to introduce DNA into a wide variety of animal cells1,2. Electroporation works well with cell lines that are refractive to other techniques, such as calcium phosphate–DNA coprecipitation. But as with other transfection methods, the optimal conditions for electroporation of untested cell lines must be determined experimentally.
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页码:67 / 68
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