Development and characterization of 15 microsatellite loci for Cariniana estrellensis and transferability to Cariniana legalis, two endangered tropical tree species

被引：0
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作者：
Marcela Corbo Guidugli
Tatiana de Campos
Adna Cristina Barbosa de Sousa
Juliana Massimino Feres
Alexandre Magno Sebbenn
Moacyr Antonio Mestriner
Eucleia Primo Betioli Contel
Ana Lilia Alzate-Marin
机构：
[1] Universidade de São Paulo (USP),Faculdade de Medicina de Ribeirão Preto, Departamento de Genética, Laboratório de Genética Vegetal
[2] Universidade Estadual de Campinas (Unicamp),Centro de Biologia Molecular e Engenharia Genética, Departamento de Genética e Evolução
[3] Estação Experimental de Tupi,Instituto Florestal de São Paulo
来源：
Conservation Genetics | 2009年 / 10卷
关键词：
Conservation genetics; Forest; Genetic diversity; Jequitibá branco; Molecular marker; SSR;
D O I：
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中图分类号：
学科分类号：
摘要：
From a genomic library enriched for GA/CA repeats, 15 highly polymorphic microsatellite markers were developed for Cariniana estrellensis, a tropical forest tree. The microsatellite loci were screened in 49 mature trees found between Pardo river and Mogi-Guaçu river basins, in the state of São Paulo, Brazil. A total of 140 alleles were detected with an average of 9.33 alleles per locus. The expected heterozygosity ranged from 0.37 to 0.88. These loci showed a high probability of paternity exclusion. Additionally, 12 loci were effectively transferred to Cariniana legalis. High levels of polymorphism make the present SSR markers useful for population genetic studies.
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