Selection of Lactobacillus plantarum TN627 as a new probiotic candidate based on in vitro functional properties

被引:0
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作者
Wacim Bejar
Ameny Farhat-Khemakhem
Slim Smaoui
Mohamed Makni
Mounira Ben Farhat
Badis Abdelmalek
Lotfi Mellouli
Emmanuelle Maguin
Samir Bejar
Hichem Chouayekh
机构
[1] Université de Sfax,Laboratoire de Microorganismes et de Biomolécules (LMB), Centre de Biotechnologie de Sfax (CBS)
[2] Faculté des Sciences de Sfax,Laboratoire de Physiologie Animale
[3] Université Saad Dahlab de Blida,Pédagogique de Biochimie et Microbiologie Industrielle (LPBMI), Département de Chimie Industrielle
[4] I.N.R.A.,Unité de Génétique Microbienne, UR895
来源
Biotechnology and Bioprocess Engineering | 2011年 / 16卷
关键词
TN627; probiotic potential; antibacterial activity; sensitivity to antibiotics; adhesion capacity;
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学科分类号
摘要
Nine lactobacilli previously selected for high antagonism against food borne bacterial pathogens were identified via 16S rRNA gene sequencing and screened for probiotic potential for use in poultry production. The lactobacilli were subjected to a subtractive in vitro analysis system using a certified probiotic as reference. This allowed for selection of a milk-derived Lactobacillus plantarum strain, termed TN627. This organic acid-producing bacterium was free of harmful enzymatic activity and sensitive to several antibiotics. It also showed good growth at pH 4 and in the presence of bile. L. plantarum TN627 also exhibited high efficacy of adhesion to chicken enterocytes, which correlated with detecting genes encoding the mucusbinding, adhesion-promoting proteins (Mub and MapA) and the adhesion-like factor EF-Tu, commonly involved in adherence of lactobacilli to mucosal surfaces. Taken together, our findings suggest that TN627 is a promising probiotic candidate with high potential for application as a supplement in the animal feed industry.
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页码:1115 / 1123
页数:8
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