A new murine osteoblastic cell line immortalized with the SV40 large T antigen

被引:0
|
作者
Miki Nakamura-Ota
Ryoji Hamanaka
Hiroyuki Yano
Sawako Adachi
Hideaki Sumiyoshi
Noritaka Matsuo
Hidekatsu Yoshioka
机构
[1] Oita University,Department of Matrix Medicine, Faculty of Medicine
[2] Oita University,Department of Cell Biology, Faculty of Medicine
[3] Oita University,Division of Radioisotope Research, Department of Research Support
来源
Cell and Tissue Banking | 2014年 / 15卷
关键词
Bone formation; Cell culture; MC3T3-E1 cell; Osteoblastic cell line; SV40 large T antigen;
D O I
暂无
中图分类号
学科分类号
摘要
The murine preosteoblastic cell line, MC3T3-E1, is widely used to study bone formation and differentiation in vitro. However, this cell line is unstable in culture. The current study was designed to establish a stable osteoblastic cell line. A mammalian expression vector carrying the SV 40 large T antigen was introduced into a primary culture of cells isolated from the calvaria of newborn mice. Among isolated cell lines, the MN16 cell line was selected for further characterization. The MN16 cell line was cultured for 28 days, and compared with the MC3T3-E1 cell line with or without induction. The expression of bone-related genes was examined using the real-time RT-PCR technique. Alizarin red and von Kossa staining were used to detect mineralization of nodules in the cultures. The cell line showed the characteristics of osteoblastic cells in term of gene expression patterns of various molecular markers and calcium deposition in the cell layer after induction. Furthermore, the MN16 cells showed strong adhesion to the basic domain of collagen, a result that is specific for bone-derived cells. The MN16 cell line was found to be stably differentiated into bone formation cells in vitro and should be useful for studying bone biology.
引用
收藏
页码:373 / 380
页数:7
相关论文
共 50 条
  • [41] SV40 large T antigen-specific human T cell memory responses
    Coleman, Sharon
    Gibbs, Allen
    Butchart, Eric
    Mason, Malcolm D.
    Jasani, Bharat
    Tabi, Zsuzsanna
    JOURNAL OF MEDICAL VIROLOGY, 2008, 80 (08) : 1497 - 1504
  • [42] Establishment and characterization of an SV40 immortalized chicken intestinal epithelial cell line
    Ghiselli, Federico
    Felici, Martina
    Piva, Andrea
    Grilli, Ester
    POULTRY SCIENCE, 2023, 102 (10)
  • [43] ANALYSIS OF MECHANISMS CONTROLLING THE INTERACTIONS OF SV40 LARGE T-ANTIGEN WITH THE SV40 ORI REGION
    SCHIRMBECK, R
    DEPPERT, W
    VIROLOGY, 1988, 165 (02) : 527 - 538
  • [44] Inhibition of SV40 large T antigen induced apoptosis by small T antigen
    T Kolzau
    R S Hansen
    D Zahra
    R R Reddel
    A W Braithwaite
    Oncogene, 1999, 18 : 5598 - 5603
  • [45] Inhibition of SV40 large T antigen induced apoptosis by small T antigen
    Kolzau, T
    Hansen, RS
    Zahra, D
    Reddel, RR
    Braithwaite, AW
    ONCOGENE, 1999, 18 (40) : 5598 - 5603
  • [46] Physiological relevance of mouse cholangiocytes immortalized by transfection of SV40 T antigen gene
    Mano, Y
    Ishii, M
    Kisara, N
    Kobayashi, Y
    Fukushima, K
    Ueno, Y
    Toyota, T
    HEPATOLOGY, 1998, 28 (04) : 536A - 536A
  • [47] Cell density-dependent related gene expression: SV40 large T antigen levels in immortalized astrocyte lines.
    Frisa, PS
    Jacobberger, JW
    BMC CELL BIOLOGY, 2002, 3 (1)
  • [48] Characterization of immortalized human umbilical and iliac vein endothelial cell lines after transfection with SV40 large T-antigen
    van Leeuwen, EBM
    Veenstra, R
    van Wijk, R
    Molema, G
    Hoekstra, A
    Ruiters, MHJ
    van der Meer, J
    BLOOD COAGULATION & FIBRINOLYSIS, 2000, 11 (01) : 15 - 25
  • [49] Establishment of SV40 large T antigen-immortalized bovine liver sinusoidal cell lines and their immunological responses to deoxynivalenol and lipopolysaccharide
    Yoshioka, Miyako
    Takenouchi, Takato
    Kitani, Hiroshi
    Okada, Hiroyuki
    Yamanaka, Noriko
    CELL BIOLOGY INTERNATIONAL, 2016, 40 (12) : 1372 - 1379
  • [50] SV40 T-ANTIGEN AND CELL-TRANSFORMATION
    GRAESSMANN, M
    GRAESSMANN, A
    CELL BIOLOGY INTERNATIONAL REPORTS, 1983, 7 (07) : 511 - 511