Effects of VEGF and FGF-2 on proliferation and differentiation of human periodontal ligament stem cells

被引:0
|
作者
Joo-Hee Lee
Soyoun Um
Jun-Hyeog Jang
Byoung Moo Seo
机构
[1] Seoul National University,Biotooth Engineering Lab, Department of Oral and Maxillofacial Surgery, School of Dentistry, BK21, Craniomaxillofacial Life Science, Dental Research Institute
[2] Inha University,Department of Biochemistry, School of Medicine
[3] Seoul National University School of Dentistry,Department of Oral and Maxillofacial Surgery
来源
Cell and Tissue Research | 2012年 / 348卷
关键词
Periodontal ligament stem cells; Odonto-/osteogenic differentiation; Vascular endothelial growth factor; Fibroblast growth factor-2; Tissue regeneration;
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摘要
Human periodontal ligament stem cells (PDLSCs) from extracted third molar teeth are a type of adult stem cell originating from dental tissue. PDLSCs are known to have a self-renewal capacity and multi-lineage differentiation potential. Vascular endothelial growth factor (VEGF), an angiogenic/vasculogenic factor, has been shown to stimulate endothelial cell mitogenesis and cell migration. Another growth factor, fibroblast growth factor-2 (FGF-2), a mitogenic factor, enhances osteogenesis in mesenchymal stem cells (MSCs). This study examines the effects of VEGF and FGF-2 on PDLSCs in vitro and in vivo compared with those on bone marrow stem cells (BMSCs) as a positive control. Treatment of PDLSCs with VEGF increases the accumulation of calcium nodules, alkaline phosphatase (ALP) activity and the formation of hard tissue and up-regulates the mRNA level of runt-related transcription factor 2 (Runx2). In contrast, FGF-2 enhances the proliferation of PDLSCs in vitro in cell culture, where it significantly decreases calcium accumulation and ALP activity and down-regulates the expression of osteogenic gene markers (i.e., Runx2, ALP, type I collagen) involved in osteogenic induction. We have also transplanted PDLSCs with hydroxyapatite/tricalcium phosphate particles (HA/TCP) as carriers for each factor (VEGF, FGF-2) into nude mice and, after 8 weeks, observed the in vivo formation of hard tissue at the dorsal surface. Based on our results, we suggest that VEGF has positive effects on odonto-/osteogenic differentiation in vitro and on the formation of mineralized structure in vivo. FGF-2 might be a powerful promoter of the proliferation of progenitor cells in hard tissue regeneration but exogenous FGF-2 might inhibit terminal differentiation.
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页码:475 / 484
页数:9
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