Determination of hemoglobin in plasma and serum by linear-sweep polarography

被引：0
|
作者：
Jun-feng Song
Xiao-feng Kang
Wei Guo
机构：
[1] Institute of Electroanalytical Chemistry,
[2] Northwest University,undefined
[3] Xi’an 710069,undefined
[4] P.R. China,undefined
[5] Department of Chemistry,undefined
[6] Bao ji Art and Science College,undefined
[7] Bao ji 721007,undefined
[8] P.R. China,undefined
来源：
Fresenius' Journal of Analytical Chemistry | 1997年 / 357卷
关键词：
H2O2; Microtitre Plate; Serum Protein; Good Reproducibility; Calibration Plot;
D O I：
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中图分类号：
学科分类号：
摘要：
A new method for the determination of hemoglobin (Hb) is described. It is based on the amplification of the Hb enzyme-catalyzed reaction between O-phenyldiamine (OPD) and H2O2 and polarographic measurement of the enzyme-generated product 2,2′-diaminoazobenzene (DAA). A linear calibration plot has been obtained within the range of 1∼20 μg/ml (r = 0.987) Hb. The assay is performed on the microtitre plate to avoid the influence of serum protein and to obtain good reproducibility. The method is practical, reliable and convenient, and has been applied to determine Hb in plasma and serum.
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页码：127 / 129
页数：2
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