Purinergic Modulation of Na+,K+,Cl− Cotransport and MAP Kinases is Limited to C11-MDCK Cells Resembling Intercalated Cells from Collecting Ducts

被引：0
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作者：
S.N. Orlov
N.O. Dulin
F. Gagnon
M. Gekle
J.G. Douglas
J.H. Schwartz
P. Hamet
机构：
[1] Centre de recherche du CHUM,
[2] University of Montreal,undefined
[3] Montreal,undefined
[4] Quebec,undefined
[5] Canada,undefined
[6] Cardiovascular Center,undefined
[7] Medical College of Wisconsin,undefined
[8] Milwaukee,undefined
[9] WI,undefined
[10] USA,undefined
[11] Department of Physiology,undefined
[12] University of Wurzburg,undefined
[13] Germany,undefined
[14] School of Medicine,undefined
[15] Case Western University and University Hospitals of Cleveland,undefined
[16] OH,undefined
[17] USA,undefined
[18] Boston University Medical Center,undefined
[19] MA,undefined
[20] USA,undefined
来源：
The Journal of Membrane Biology | 1999年 / 172卷
关键词：
Key words: Na+,K+,Cl− cotransport — P2-purinoceptors — MAP kinases — Renal epithelium;
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学科分类号：
摘要：
We demonstrated recently that in renal epithelial cells from collecting ducts of Madin-Darby canine kidneys (MDCK), Na+,K+,Cl− cotransport is inhibited up to 50% by ATP via its interaction with P2Y purinoceptors (Biochim. Biophys. Acta 1998. 1369:233–239). In the present study we examined which type of renal epithelial cells possesses the highest sensitivity of Na+,K+,Cl− cotransport to purinergic regulation. We did not observe any effect of ATP on Na+,K+,Cl− cotransport in renal epithelial cells from proximal and distal tubules, whereas in renal epithelial cells from rabbit and rat collecting ducts ATP decreased the carrier's activity by ∼30%. ATP did not affect Na+,K+,Cl− cotransport in C7 subtype MDCK cells possessing the properties of principal cells but led to ∼85% inhibition of this carrier in C11-MDCK cells in which intercalated cells are highly abundant. Both C7- and C11-MDCK exhibited ATP-induced IP3 and cAMP production and transient elevation of [Ca2+]i. In contrast to the above-listed signaling systems, ATP-induced phosphorylation of ERK and JNK MAP kinases was observed in C11-MDCK only. Thus, our results reveal that regulation of renal Na+,K+,Cl− cotransport by P2Y receptors is limited to intercalated cells from collecting ducts and indicate the involvement of the MAP kinase cascade in purinergic control of this ion carrier's activity.
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页码：225 / 234
页数：9
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