Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1

被引：0

作者：

Dalibor Milić

Markus Dick

Daniel Mulnaes

Christopher Pfleger

Anna Kinnen

Holger Gohlke

Georg Groth

机构：

[1] Heinrich Heine University Düsseldorf,Institute of Biochemical Plant Physiology and Bioeconomy Science Center (BioSC)

[2] Heinrich Heine University Düsseldorf,Institute of Pharmaceutical and Medicinal Chemistry and Bioeconomy Science Center (BioSC)

[3] Forschungszentrum Jülich GmbH,John von Neumann Institute for Computing (NIC), Jülich Supercomputing Centre (JSC) & Institute for Complex Systems

[4] Max F. Perutz Laboratories, Structural Biochemistry (ICS 6)

[5] University of Vienna,Department of Structural and Computational Biology

[6] Vienna Biocenter,Division of Chemistry and Chemical Engineering

[7] California Institute of Technology,undefined

[8] Pasadena,undefined

来源：

Scientific Reports | / 8卷

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摘要：

Synthetic peptides derived from ethylene-insensitive protein 2 (EIN2), a central regulator of ethylene signalling, were recently shown to delay fruit ripening by interrupting protein–protein interactions in the ethylene signalling pathway. Here, we show that the inhibitory peptide NOP-1 binds to the GAF domain of ETR1 – the prototype of the plant ethylene receptor family. Site-directed mutagenesis and computational studies reveal the peptide interaction site and a plausible molecular mechanism for the ripening inhibition.

引用

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