Genetic markers for search of rhizobia based on symbiotic genes

被引:0
|
作者
E. S. Akimova
R. S. Gumenko
Z. R. Vershinina
Al. Kh. Baymiev
An. Kh. Baymiev
机构
[1] Ufa Scientific Centre Russian Academy of Sciences,Institute of Biochemistry and Genetics
来源
Microbiology | 2017年 / 86卷
关键词
rhizobia; legumes; symbiotic genes;
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中图分类号
学科分类号
摘要
The possible application of rhizobial symbiotic genes as markers for the search and primary identification of rhizobia from temperate-zone legumes was studied. It was shown that conservative sym genes nifH and nifD could be used as markers for rapid search of rhizobia among the analyzed isolates, while more variable genes nifK and nodC could be used for their primary identification. Efficiency of the proposed method was shown in analysis of bacterial isolates obtained from Onobrychis arenaria and Astragalus cicer root nodules.
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页码:640 / 646
页数:6
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