High-throughput assay of (R)-phenylacetylcarbinol synthesized by pyruvate decarboxylase

被引：0

作者：

Michael Breuer

Martina Pohl

Bernhard Hauer

Bettina Lingen

机构：

[1] BASF AG,

[2] GVF/E-A030,undefined

[3] 67056 Ludwigshafen,undefined

[4] Germany,undefined

[5] MBP Cologne GmbH,undefined

[6] Neurather Ring 1,undefined

[7] 51063 Köln,undefined

[8] Germany,undefined

[9] Institut für Enzymtechnologie der Heinrich-Heine-Universität Düsseldorf,undefined

[10] im Forschungszentrum Jülich,undefined

[11] 52426 Jülich,undefined

[12] Germany,undefined

来源：

Analytical and Bioanalytical Chemistry | 2002年 / 374卷

关键词：

High-throughput assay (R)-Phenylacetylcarbinol Pyruvate decarboxylase Tetrazolium red;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A novel assay has been developed for the detection of (R)-phenylacetylcarbinol, (R)-PAC, a chiral intermediate in the industrial synthesis of ephedrine. It is the product of a biotransformation of benzaldehyde catalysed by the enzyme pyruvate decarboxylase. The assay, using 2,3,5-triphenyltetrazolium chloride, enables high-throughput photometric analysis of the activity of the enzyme thus avoiding time-consuming chromatographic procedures.

引用

页码：1069 / 1073

页数：4

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