High-throughput assay of (R)-phenylacetylcarbinol synthesized by pyruvate decarboxylase

被引：0
|
作者：
Michael Breuer
Martina Pohl
Bernhard Hauer
Bettina Lingen
机构：
[1] BASF AG,
[2] GVF/E-A030,undefined
[3] 67056 Ludwigshafen,undefined
[4] Germany,undefined
[5] MBP Cologne GmbH,undefined
[6] Neurather Ring 1,undefined
[7] 51063 Köln,undefined
[8] Germany,undefined
[9] Institut für Enzymtechnologie der Heinrich-Heine-Universität Düsseldorf,undefined
[10] im Forschungszentrum Jülich,undefined
[11] 52426 Jülich,undefined
[12] Germany,undefined
来源：
Analytical and Bioanalytical Chemistry | 2002年 / 374卷
关键词：
High-throughput assay (R)-Phenylacetylcarbinol Pyruvate decarboxylase Tetrazolium red;
D O I：
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中图分类号：
学科分类号：
摘要：
A novel assay has been developed for the detection of (R)-phenylacetylcarbinol, (R)-PAC, a chiral intermediate in the industrial synthesis of ephedrine. It is the product of a biotransformation of benzaldehyde catalysed by the enzyme pyruvate decarboxylase. The assay, using 2,3,5-triphenyltetrazolium chloride, enables high-throughput photometric analysis of the activity of the enzyme thus avoiding time-consuming chromatographic procedures.
引用
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页码：1069 / 1073
页数：4
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