Rhizobium capsici sp. nov., isolated from root tumor of a green bell pepper (Capsicum annuum var. grossum) plant

被引:0
|
作者
Shih-Yao Lin
Mei-Hua Hung
Asif Hameed
You-Cheng Liu
Yi-Han Hsu
Cheng-Zhe Wen
A. B. Arun
Hans-Jürgen Busse
Stefanie P. Glaeser
Peter Kämpfer
Chiu-Chung Young
机构
[1] National Chung Hsing University,Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources
[2] National Chung Hsing University,Bachelor Program of Bio
[3] Yenepoya University,Industry Management, College of Agriculture and Natural Resources
[4] Veterinärmedizinische Universität,Yenepoya Research Center
[5] Justus-Liebig-Universität Giessen,Institut für Bakteriologie, Mykologie und Hygiene
[6] National Chung Hsing University,Institut für Angewandte Mikrobiologie
来源
Antonie van Leeuwenhoek | 2015年 / 107卷
关键词
Root tumors; var. ; Legumes; Housekeeping gene; sp. nov;
D O I
暂无
中图分类号
学科分类号
摘要
A novel, Gram-staining-negative, rod-shaped, aerobic and motile bacterium, designated strain CC-SKC2T, was isolated from the root tumor of a green bell pepper (Capsicum annuum var. grossum) plant in Taiwan. Cells were positive for oxidase and catalase activities and exhibited growth at 25–37 °C, pH 4.0–9.0 and tolerated NaCl concentrations up to 4.0 % (w/v). Strain CC-SKC2T is able to trigger nodulation in soybean (Glycine max Merr.), but not in Capsicum annuum var. grossum, red bean (Vigna angularis), sesbania (Sesbania roxburghii Merr.) or alfalfa (Medicago varia Martin.). The novel strain shared highest 16S rRNA gene sequence similarity to Rhizobium rhizoryzae KCTC 23652T and Rhizobium straminoryzae CC-LY845T (both 97.5 %) followed by Rhizobium lemnae L6-16T (97.3 %), Rhizobium pseudoryzae KCTC 23294T (97.1 %), and Rhizobium paknamense NBRC 109338T (97.0 %), whereas other Rhizobium species shared <96.7 % similarity. The DNA–DNA relatedness values of strain CC-SKC2T with R. rhizoryzae KCTC 23652T, R. pseudoryzae KCTC 23294T and R. paknamense NBRC 109338T were 11.4, 17.2 and 17.0 %, respectively (reciprocal values were 11.1, 28.3 and 24.0 %, respectively). Phylogenetic analysis based on 16S rRNA, atpD and recA genes revealed a distinct taxonomic position attained by strain CC-SKC2T with respect to other Rhizobium species. The major fatty acids in strain CC-SKC2T were C16:0, C19:0 cyclo ω8c, C14:0 3-OH and/or C16:1 iso I and C18:1ω7c and/or C18:1ω6c. The polyamine pattern showed predominance of spermidine and moderate amounts of sym-homospermidine. The predominant quinone system was ubiquinone (Q-10) and the DNA G+C content was 60.5 mol%. On the basis of polyphasic taxonomic evidence presented here, strain CC-SKC2T is proposed to represent a novel species within the genus Rhizobium, for which the name Rhizobium capsici sp. nov. is proposed. The type strain is CC-SKC2T (=BCRC 80699T = JCM 19535T).
引用
收藏
页码:773 / 784
页数:11
相关论文
共 50 条
  • [21] Pseudomonas capsici sp. nov., a plant-pathogenic bacterium isolated from pepper leaf in Georgia, USA
    Zhao, Mei
    Koirala, Santosh
    Chen, Hsiao-Chun
    Gitaitis, Ronald
    Kvitko, Brian
    Dutta, Bhabesh
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2021, 71 (08)
  • [22] Rhizobium halophytocola sp nov., isolated from the root of a coastal dune plant
    Bibi, Fehmida
    Chung, Eu Jin
    Khan, Ajmal
    Jeon, Che Ok
    Chung, Young Ryun
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2012, 62 : 1997 - 2003
  • [23] Rhizobium alarense sp. nov. and Rhizobium halophilum sp. nov. isolated from the nodule and rhizosphere of Lotus japonicus
    Hongling Shen
    Xiaoxia Luo
    Zhanfeng Xia
    Chuanxing Wan
    Archives of Microbiology, 2022, 204
  • [24] Rhizobium tubonense sp. nov., isolated from root nodules of Oxytropis glabra
    Zhang, Rong Juan
    Hou, Bao Chao
    Wang, En Tao
    Li, Ying, Jr.
    Zhang, Xiao Xia
    Chen, Wen Xin
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2011, 61 : 512 - 517
  • [25] Rhizobium alarense sp. nov. and Rhizobium halophilum sp. nov. isolated from the nodule and rhizosphere of Lotus japonicus
    Shen, Hongling
    Luo, Xiaoxia
    Xia, Zhanfeng
    Wan, Chuanxing
    ARCHIVES OF MICROBIOLOGY, 2022, 204 (11)
  • [26] Functional properties of protein isolates from bell pepper (Capsicum annuum L. var. annuum) seeds
    Li, Mo
    Wen, Xin
    Peng, Yu
    Wang, Yuxiao
    Wang, Kunli
    Ni, Yuanying
    LWT-FOOD SCIENCE AND TECHNOLOGY, 2018, 97 : 802 - 810
  • [27] Rhizobium puerariae sp nov., an endophytic bacterium from the root nodules of the medicinal plant Pueraria candollei var. candollei
    Boonsnongcheep, Panitch
    Prathanturarug, Sompop
    Takahashi, Yoko
    Matsumoto, Atsuko
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2016, 66 : 1236 - 1241
  • [28] GENOTOXICITY AND ANTIOXIDANT ENZYME ACTIVITIES INDUCED BY THE CAPTAN FUNGICIDE IN THE ROOT OF BELL PEPPER (Capsicum annuum L. var. grossum L. cv. Kandil)
    Seyhan, Muge
    Yuzbasioglu, Elif
    Dalyan, Eda
    Akpinar, Ilgin
    Unal, Muammer
    TRAKYA UNIVERSITY JOURNAL OF NATURAL SCIENCES, 2019, 20 (02) : 97 - 103
  • [29] Rhizobium chutanense sp. nov., isolated from root nodules of Phaseolus vulgaris in China
    Huo, Yunyun
    Tong, Wenjun
    Wang, Juanjuan
    Wang, Fang
    Bai, Wenqing
    Wang, Entao
    Shi, Peng
    Chen, Weimin
    Wei, Gehong
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2019, 69 (07) : 2049 - 2056
  • [30] Janibacter endophyticus sp. nov., an Endophytic Actinobacterium Isolated from the Root of Paris polyphylla Smith var. Yunnanensis
    Zhang, Zhen
    Zhou, En-Min
    Li, Cong-Jian
    Jiang, Xing-Wang
    Mao, Rui-Feng
    Liu, Jing-Ran
    Zhi, Xiao-Yang
    Yang, Ling-Ling
    CURRENT MICROBIOLOGY, 2022, 79 (02)