Two novel extracellular cholesterol oxidases of Bacillus sp. isolated from fermented flatfish

被引：0
|
作者：
Chang-Ho Rhee
Kwan-Pil Kim
Heui-Dong Park
机构：
[1] National University,Department of Food Science and Technology, Kyungpook
来源：
Biotechnology Letters | 2002年 / 24卷
关键词：
sp.; cholesterol oxidase; fermented flatfish; purification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Two novel extracellular cholesterol oxidases designated CO1 and CO2, from Bacillus sp. SFF34, were purified 5.6 and 5.9-fold giving Mr values of 36 and 37 kDa. The optimum temperature for the activity was 60 °C (CO1) and 40 °C (CO2), and the optimum pH was 6.25 (CO1) and 6 (CO2) over 30 min reaction time. The apparent Km values for cholesterol were 6.76 mM (CO1) and 4.50 mM (CO2). Both the enzymes could oxidize 5α-cholestane, 5α-cholestane-3β-ol-7-one, coprostane, dihydrocholesterol, hecogenin, β-sitosterol and stigmasterol.
引用
收藏
页码：1385 / 1389
页数：4
相关论文
共 50 条
  • [31] Production and Partial Characterization of Extracellular Protease from Bacillus sp. GJP2 Isolated from a Hot Spring
    Jugran J.
    Joshi G.K.
    Bhatt J.P.
    Shanker A.
    Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 2016, 86 (1) : 171 - 178
  • [32] A novel textile dye degrading extracellular laccase from symbiotic bacterium of Bacillus sp. CF96 isolated from gut termite (Anacanthotermes)
    Javadzadeh, Seyedeh-Golnaz
    Asoodeh, Ahmad
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 145 : 355 - 363
  • [33] Bacillus paralicheniformis sp nov., isolated from fermented soybean paste
    Dunlap, Christopher A.
    Kwon, Soon-Wo
    Rooney, Alejandro P.
    Kim, Soo-Jin
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2015, 65 : 3487 - 3492
  • [34] Bacillus glycinifermentans sp nov., isolated from fermented soybean paste
    Kim, Soo-Jin
    Dunlap, Christopher A.
    Kwon, Soon-Wo
    Rooney, Alejandro P.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2015, 65 : 3586 - 3590
  • [35] Extracellular Overexpression of Chitosanase from Bacillus sp. TS in Escherichia coli
    Zhanping Zhou
    Shuangzhi Zhao
    Shouquan Wang
    Xiaoman Li
    Li Su
    Yanhe Ma
    Jian Li
    Jiangning Song
    Applied Biochemistry and Biotechnology, 2015, 175 : 3271 - 3286
  • [36] Production and partial characterization of extracellular glucose isomerase using thermophilic Bacillus sp. isolated from agriculture land
    Sukumar, MohanaSundaram
    Jeyaseelan, Arun
    Sivasankaran, Thirumalai
    Mohanraj, Pennarasi
    Mani, Prasanna
    Sudhakar, Gowthamraj
    Arumugam, Vimalraj
    Bakthavachalu, Sasirekha
    Ganeshan, Alagulakshmi
    Susee, Monika
    BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2013, 2 (01) : 45 - 49
  • [37] A novel extracellular α-amylase from a new isolated Bacillus sp WHO:: cloning, purification and biochemical characterization
    Safari, M. Ghanbari
    Khajeh, K.
    Ghollasi, M.
    Naderi-Manesh, H.
    FEBS JOURNAL, 2008, 275 : 402 - 402
  • [38] Biodegradation of crystal violet by an isolated Bacillus sp.
    Ayed, Lamia
    Cheriaa, Jihane
    Laadhari, Neji
    Cheref, Abdelkarim
    Bakhrouf, Amina
    ANNALS OF MICROBIOLOGY, 2009, 59 (02) : 267 - 272
  • [39] A novel organic-solvent and detergent resistant esterase from Bacillus sp. isolated from Bazangan Lake
    Pasban-Ziyarat, Fatemeh
    Mehrzad, Jamshid
    Asoodeh, Ahmad
    Deiminiat, Behjat
    Motavalizadehkakhky, Alireza
    BIOCATALYSIS AND BIOTRANSFORMATION, 2022, 40 (02) : 121 - 132
  • [40] Characterization of a Bacillus sp. isolated from fermented food and its synbiotic effect with barley -glucan as a biocontrol agent in the aquaculture industry
    Lee, Jong Min
    Jang, Won Je
    Hasan, Md Tawheed
    Lee, Bong-Joo
    Kim, Kang Woong
    Lim, Sang Gu
    Han, Hyon-Sob
    Kong, In-Soo
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 103 (03) : 1429 - 1439
12345