Two novel extracellular cholesterol oxidases of Bacillus sp. isolated from fermented flatfish

被引：0
|
作者：
Chang-Ho Rhee
Kwan-Pil Kim
Heui-Dong Park
机构：
[1] National University,Department of Food Science and Technology, Kyungpook
来源：
Biotechnology Letters | 2002年 / 24卷
关键词：
sp.; cholesterol oxidase; fermented flatfish; purification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Two novel extracellular cholesterol oxidases designated CO1 and CO2, from Bacillus sp. SFF34, were purified 5.6 and 5.9-fold giving Mr values of 36 and 37 kDa. The optimum temperature for the activity was 60 °C (CO1) and 40 °C (CO2), and the optimum pH was 6.25 (CO1) and 6 (CO2) over 30 min reaction time. The apparent Km values for cholesterol were 6.76 mM (CO1) and 4.50 mM (CO2). Both the enzymes could oxidize 5α-cholestane, 5α-cholestane-3β-ol-7-one, coprostane, dihydrocholesterol, hecogenin, β-sitosterol and stigmasterol.
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页码：1385 / 1389
页数：4
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