Mitochondrial DNA in human hair shafts – existence of intra-individual differences?

被引：0
|
作者：
J. Hühne
H. Pfeiffer
K. Waterkamp
B. Brinkmann
机构：
[1] Institute of Forensic Medicine,
[2] University Münster,undefined
[3] von Esmarch Strasse 62,undefined
[4] D-48149 Münster,undefined
[5] Germany,undefined
来源：
International Journal of Legal Medicine | 1999年 / 112卷
关键词：
Key words Mitochondrial DNA; Hypervariable region 1; DNA extraction; Human hair shafts; Heteroplasmy;
D O I：
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中图分类号：
学科分类号：
摘要：
The sequences of the hypervariable region 1 (HV1) of the mitochondrial DNA control region from multiple hair shafts from 10 unrelated individuals were compared to determine the frequency of differences in hairs from one individual. The extraction method described herein showed an average success rate of 67% for all 150 hair shafts tested in HV1. The mtDNA sequences from the hair shafts matched the sequences from the corresponding blood and saliva samples taken from the same donor and no evidence of heteroplasmy was found. The results emphasize the reliability of DNA extraction and mtDNA typing from human hair shafts for forensic purposes.
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页码：172 / 175
页数：3
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