Lysobacter agri sp. nov., a bacterium isolated from soil

A bacterial strain, designated as THG-SKA3T, was isolated from field soil of Kyung Hee University, South Korea. Cells of the isolate were observed to be Gram-negative, aerobic, rod-shaped and motile by gliding. The strain was found to grow optimally at 28 °C, at pH 7 and in absence of NaCl. Based on 16S rRNA gene sequence comparisons, strain THG-SKA3T shared highest sequence similarity with Lysobacter niastensis KACC 11588T followed by Lysobacter panacisoli KACC 17502T, Lysobacter enzymogenes LMG 8762T and Lysobacter oryzae KCTC 22249T. The G+C content of THG-SKA3T was determined to be 68.9 mol%. The DNA–DNA relatedness values between strain THG-SKA3T and its closest phylogenetic neighbors were below 25.0 %.The major polar lipids of strain THG-SKA3T were determined to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was identified as ubiquinone 8 (Q-8). The major cellular fatty acids were identified as branched chain iso-C15:0, iso-C16:0 and unsaturated iso-C17:1ω9c. On the basis of polyphasic data presented, it is evident that strain THG-SKA3T represents a novel species of the genus Lysobacter, for which the name Lysobacter agri sp. nov. (type strain THG-SKA3T = KACC 18283T = CSCTCC AB 2015126T) is proposed.