Low population genetic differentiation in two Tamarix species (Tamarix austromongolica and Tamarix chinensis) along the Yellow River

Geological events have been shown to be the main factors affecting the distributions and population genetic structure of species. However, the impact of the Yellow River, the second longest river in China, on the distribution and genetic structure of the endemic flora remains largely unknown. Here, we used microsatellites to evaluate the genetic structure of Tamarix austromongolica and Tamarix chinensis (343 individuals in total), two endemic tree species widely distributed along the Yellow River, as well as the role of river drainages in shaping the structure. We found that frequent gene flow resulted in low genetic differentiation among populations within species (T. austromongolica: Fst = 0.144, Nm = 1.486; T. chinensis: Fst = 0.103, Nm = 2.177); and low levels of genetic differentiation were detected between populations within species on the north and south banks. Also, high gene flow was found between populations in the upper reaches and those in the middle reaches, and middle reaches and lower reaches of the Yellow River (T. austromongolica: Fst = 0.023, Nm = 10.619; T. chinensis: Fst = 0.045, Nm = 5.306). Additionally, discriminant analysis of principal components (DAPC) and Bayesian analysis revealed a weak population structure in T. austromongolica and T. chinensis. Therefore, the Yellow River does not act as a barrier to dispersal and gene flow for Tamarix. Our findings provide support for the role of the river in shaping the spatial distribution and the genetic structure of species.