Two-color, two-photon uncaging of glutamate and GABA

被引：0
|
作者：
Kantevari S. [1 ]
Matsuzaki M. [2 ]
Kanemoto Y. [2 ]
Kasai H. [2 ]
Ellis-Davies G.C.R. [1 ]
机构：
[1] Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, PA
[2] Laboratory of Structural Physiology, Graduate School of Medicine, University of Tokyo, Tokyo
来源：
Nature Methods | 2010年 / 7卷 / 2期
基金：
美国国家卫生研究院; 日本科学技术振兴机构;
关键词：
D O I：
10.1038/nmeth.1413
中图分类号：
学科分类号：
摘要：
We developed a caged GABA (γ-aminobutyric acid), which, when combined with an appropriate caged glutamate, allows bimodal control of neuronal membrane potential with subcellular resolution using optically independent two-photon uncaging of each neurotransmitter. We used two-color, two-photon uncaging to fire and block action potentials from rat hippocampal CA1 neurons in brain slices with 720-nm and 830-nm light, respectively. Our method should be generalizable to other chemical messenger pairs. © 2010 Nature America, Inc. All rights reserved.
引用
收藏
页码：123 / 125
页数：2
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