High-Throughput DNA Microarray Detection of Pathogenic Bacteria in Shallow Well Groundwater in the Kathmandu Valley, Nepal

被引:0
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作者
Daisuke Inoue
Takuji Hinoura
Noriko Suzuki
Junqin Pang
Rabin Malla
Sadhana Shrestha
Saroj Kumar Chapagain
Hiroaki Matsuzawa
Takashi Nakamura
Yasuhiro Tanaka
Michihiko Ike
Kei Nishida
Kazunari Sei
机构
[1] Kitasato University,Department of Health Science, School of Allied Health Sciences
[2] Kitasato University,Environment and Medical Sciences Course, Graduate School of Medical Sciences
[3] Osaka University,Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering
[4] Center of Research for Environment Energy and Water (CREEW),Interdisciplinary Graduate School of Medicine and Engineering
[5] University of Yamanashi,International Research Center for River Basin Environment
[6] University of Yamanashi,undefined
来源
Current Microbiology | 2015年 / 70卷
关键词
Groundwater Sample; Pathogenic Bacterium; Arthrobacter; Total Coliform; Polymerase Chain Reaction Mixture;
D O I
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中图分类号
学科分类号
摘要
Because of heavy dependence on groundwater for drinking water and other domestic use, microbial contamination of groundwater is a serious problem in the Kathmandu Valley, Nepal. This study investigated comprehensively the occurrence of pathogenic bacteria in shallow well groundwater in the Kathmandu Valley by applying DNA microarray analysis targeting 941 pathogenic bacterial species/groups. Water quality measurements found significant coliform (fecal) contamination in 10 of the 11 investigated groundwater samples and significant nitrogen contamination in some samples. The results of DNA microarray analysis revealed the presence of 1–37 pathogen species/groups, including 1–27 biosafety level 2 ones, in 9 of the 11 groundwater samples. While the detected pathogens included several feces- and animal-related ones, those belonging to Legionella and Arthrobacter, which were considered not to be directly associated with feces, were detected prevalently. This study could provide a rough picture of overall pathogenic bacterial contamination in the Kathmandu Valley, and demonstrated the usefulness of DNA microarray analysis as a comprehensive screening tool of a wide variety of pathogenic bacteria.
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页码:43 / 50
页数:7
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