Evaluation of metalloproteinases-2, -9, and -13 post photobiomodulation in mice talocrural joint

被引:0
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作者
Thais Fraga Abduch
Pierre Augusto Victor da Silva
Álvaro Carneiro de Souza
Lúcia Mara Januário dos Anjos
Adenilson de Souza da Fonseca
Flávia de Paoli
机构
[1] Departamento de Fisioterapia,Departamento de Fisioterapia
[2] Faculdade de Ciências Médicas e da Saúde (SUPREMA),Departamento de Morfologia, Instituto de Ciências Biológicas
[3] Centro Universitário Redentor (UniRedentor),Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcântara Gomes
[4] Universidade Federal de Juiz de Fora,Departamento de Ciências Fisiológicas, Instituto Biomédico
[5] Universidade do Estado do Rio de Janeiro,Centro de Ciências da Saúde
[6] Universidade Federal do Estado do Rio de Janeiro,undefined
[7] Centro Universitário Serra dos Órgãos,undefined
来源
Lasers in Medical Science | 2020年 / 35卷
关键词
Photobiomodulation; Low-level laser therapy; Metalloproteinase; TIMP;
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学科分类号
摘要
The extracellular matrix (ECM) is the main constituent of connective tissue with structural and regulatory functions, stimulating cell differentiation and proliferation. Moreover, ECM is a dynamic structure in the constant remodeling process, which is controlled by a balance between metalloproteinases (MMPs) and their inhibitors (TIMPs). Photobiomodulation (PBM) is widely described in the literature and applied in clinical practices, although its effects on ECM have not yet been elucidated. Therefore, it was evaluated if PBM could alter ECM components, such as MMP-2, -9, -13, and TIMP-2 from mice talocrural joints. Mice were divided into 3 groups (n = 6): control, PBM 3 J cm−2, and PBM 30 J cm−2. A low-level laser (830 nm, 10 mW, 0.05 irradiated area, energy densities 3 J cm−2 and 30 J cm−2, the irradiation time of 15 and 150 s, respectively, continuous wave) was applied on the joint for 4 consecutive days. mRNA levels of metalloproteinases genes (MMP-2, MMP-9, and MMP-13), their regulator (TIMP-2), and protein expressions of MMP-13 and TIMP-2 were quantified. PBM can alter only mRNA relative levels of MMP-2 at 30 J cm−2 (p < 0.05), while MMP-9, MMP-13, and TIMP-2 mRNA relative levels did not demonstrate statistical differences for any of the groups (p > 0.05). Regarding protein expressions, MMP-13 demonstrated positive-labeled cells, only in articular cartilage, although the cell quantification did not demonstrate statistical differences when compared with the control group (p > 0.05). TIMP-2 did not present positive-labeled cells for any tissues evaluated. Our results indicate that PBM can alter MMP-2 mRNA relative level but cannot alter MMP-9, MMP-13, and TIMP mRNA relative levels. Moreover, both MMP-13 and TIMP-2 proteins were also unaltered after PBM.
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页码:633 / 640
页数:7
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