Atomic force microscopy study of DNA conformation in the presence of drugs

被引:0
|
作者
Valeria Cassina
Davide Seruggia
Giovanni Luca Beretta
Domenico Salerno
Doriano Brogioli
Stefano Manzini
Franco Zunino
Francesco Mantegazza
机构
[1] University of Milano–Bicocca,Department of Experimental Medicine
[2] Fondation IRCCS Istituto Nazionale Tumori,Department of Experimental Oncology
[3] University of Milano–Bicocca,Department of Surgical Sciences
来源
European Biophysics Journal | 2011年 / 40卷
关键词
DNA; Atomic force microscopy; Doxorubicin; Ethidium bromide; Netropsin;
D O I
暂无
中图分类号
学科分类号
摘要
Binding of ligands to DNA gives rise to several relevant biological and biomedical effects. Here, through the use of atomic force microscopy (AFM), we studied the consequences of drug binding on the morphology of single DNA molecules. In particular, we quantitatively analyzed the effects of three different DNA-binding molecules (doxorubicin, ethidium bromide, and netropsin) that exert various pharmacologic and therapeutic effects. The results of this study show the consequences of intercalation and groove molecular binding on DNA conformation. These single-molecule measurements demonstrate morphological features that reflect the specific modes of drug–DNA interaction. This experimental approach may have implications in the design of therapeutically effective agents.
引用
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页码:59 / 68
页数:9
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