SLCO1B3 screening in colorectal cancer patients using High-Resolution Melting Analysis method and immunohistochemistry

被引:5
|
作者
Evangeli, Lampri [1 ]
Ioannis, Sainis [1 ]
Valentinos, Kounnis [1 ,2 ]
Antigony, Mitselou [4 ]
Elli, Ioachim [3 ]
Eleftheria, Hatzimichael [1 ,5 ]
Vasiliki, Galani [6 ,7 ,8 ]
Evangelos, Briasoulis [1 ,5 ]
机构
[1] Univ Ioannina, Canc Biobank Ctr, Ioannina 45500, Greece
[2] Univ Oxford, John Radcliffe Hosp, Weatherall Inst Mol Med, Hypoxia & Angiogenesis Grp,Mol Oncol Labs, Oxford, England
[3] G Hatzikosta Gen Hosp, Dept Pathol, Ioannina, Greece
[4] Univ Ioannina, Dept Forens Pathol, Ioannina, Greece
[5] Univ Ioannina, Med Sch, Dept Hematol, Ioannina, Greece
[6] Univ Ioannina, Dept Embryol, Ioannina, Greece
[7] Univ Ioannina, Dept Histol, Ioannina, Greece
[8] Univ Ioannina, Dept Anat, Ioannina, Greece
关键词
Colon cancer; HRMA; SLCO1B3; mutation detection; ORGANIC ANION TRANSPORTER; OATP1B3; 1B3; GENE; IDENTIFICATION; POLYMORPHISMS; EXPRESSION; MUTATIONS; HAPLOTYPE;
D O I
10.1177/1010428317691176
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Personalized medicine has made some major advances in colorectal cancer, but new biomarkers still remain a hot issue as an emerging tool with potential prognostic and therapeutic potential. We investigated for SLCO1B3 gene alterations and protein expression in colorectal cancer, using the novel high-resolution melting analysis technique and immunohistochemistry. Formalin-fixed paraffin-embedded tumor samples from 30 colorectal cancer patients were used. The screening for gene alterations was done by high-resolution melting analysis for all exons of SLCO1B3 gene. Organic anion-transporting polypeptide 1B3 protein expression was assessed by immunohistochemistry using the monoclonal mouse MDQ antibody. High level of polymorphism was observed in the SLCO1B3 gene. We identified three previously reported polymorphisms in exons 7, 12, and 14, 699G>A, 1557A>G, and 1833G>A, respectively. In the exon 5, one variant seems to correspond to an as yet unknown SLCO family member. The immunohistochemical study revealed that organic anion-transporting polypeptide 1B3 was expressed in 27/30 samples. Of great interest, the three samples, which were immunohistochemically negative, all appeared to accommodate mutations which lead to either early stop codons or other conformations of the tertiary protein structures affecting the antibody-epitope binding. The results of this study are of much interest as high-resolution melting analysis proved to be a reliable and rapid genotyping/scanning method for mutation detection of SLCO1B3 gene.
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页数:9
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